SARS-CoV-2 Data Analysis and Monitoring with Galaxy
training COVID-19From 2021-08-09 to 2021-08-12 - Add to your Calendar
The goal of this workshop is to build capacity in SARS-CoV-2 data analysis and data management, including data submission to ENA. After the workshop, all participants will be able to upload viral sequencing data, call all variants, create a variety of reports and create consensus alignments.
It will be a 4-day event introducing scalable and reproducible SARS-CoV-2 data analysis with Galaxy. The sessions will be pre-recorded and provided in advance.
During the workshop, there will be live support in chat and live Q&A sessions, in which experts will answer questions.
- WHEN: August 9-12, 2021
- WHO: Open for everybody, but the target audience is clinicians and researchers that deal with SARS-CoV-2 sequencing data.
- COST: Free.
- FORMAT: Virtual and asynchronous. All training sessions will be pre-recorded and provided in advance.
- SUPPORT:
- Live support in chat (Slack Channel), in which experts will answer questions on a peer-to-peer basis.
- Real-Time Q&A sessions on days 2, 3 and 4 (9 am and 4 pm CEST).
- INFRASTRUCTURE: European Galaxy server and the Galaxy Training Material. Both will stay accessible and open after the training.
- Contact us if you have questions.
Program & Material
This workshop is virtual and asynchronous. Training sessions are pre-recorded and the material provided in advance in the program below.
Whenever you’re ready to get started, you can access the material by clicking in the program on the different icons:
- for slide deck for a lecture
- for hands-on tutorial (on the Galaxy Training Material)
- for video (accompanying a lecture, a tutorial or a demo)
- for shared Galaxy history (on the European Galaxy server)
Day 1 (9.8.2021) - Introduction to Galaxy
Day 2 (10.8.2021) - Data Upload & Quality Control
Day 3 (11.8.2021) - SARS-CoV-2 Data Analysis on Public Datasets
Topic | Speaker | Material | Description | Duration |
---|---|---|---|---|
All experts | Info & Zoom link | Real-Time Q&A session (9 am CEST) | 1h | |
Galaxy for SARS-CoV-2 genome surveillance projects | Wolfgang Maier | / | Lecture: Get an overview of what day 3 has to offer: production-ready Galaxy workflows for SARS-CoV-2 sequencing data, tools you should know to automate workflow execution, and how you combine all of it to turn Galaxy into a platform for genome-surveillance. | 14m |
Variant calling, reporting, consensus building (with Galaxy GUI) | Wolfgang Maier | / Part I / Complete |
Hands-on: Illumina or ONT, ampliconic or WGS data? Learn how to combine the right set of Galaxy workflows to analyze the type of SARS-CoV-2 sequencing data of your choice. | 3h / 1h 30m |
Variant calling, reporting, consensus building (with Galaxy CLI) | Simon Bray | / | Hands-on: Learn how to use the command line to upload your SARS-CoV-2 data to a Galaxy-server and launch workflows for its analysis. Note: This first step towards automation requires the command line tool Planemo for interacting with Galaxy if you want to follow along. | 2h / 30m |
The usegalaxy.eu SARS-CoV-2 bot in action | Wolfgang Maier | Demo: See in this demo how, on usegalaxy.*, we’ve used Planemo and Bioblend to build and operate an automated SARS-CoV-2 genome surveillance system based on the Galaxy workflows for variant calling, consensus building and reporting. | 40m | |
All experts | Info & Zoom link | Real-Time Q&A session (4 pm CEST) | 1h |
Day 4 (12.8.2021) - Visualisation & Data Export
Optional extra training
Logistics
Content delivery
This is a global workshop delivered asynchronously. In practice, this means that you will have training materials available to explore them at your own pace, without any time constraints:
- Lectures: pre-recorded videos () with the theoretical explanation of the lesson, supported by slide decks ().
-
Hands-on tutorials (): a step-by-step explanation, including all the required information, to perform a data analysis, often available also as pre-recorded video ().
Most of the tutorials are developed by the Galaxy Training Network. A feedback form is available at the bottom of each tutorial page. Please fill it out, it helps us to value and improve the tutorials.
- Histories: shared Galaxy history (), on the European Galaxy server, with all that you need to reproduce what is shown in the hands-on part.
- Demo: pre-recorded videos () demonstrating a technical point or a nice feature.
Most of the material is available already, and they will all stay available after the workshop. Most of the material have been developed by a community of people via the Galaxy Training Network. Some videos were recorded for different previous events, e.g. GTN Smörgåsbord or GCC2021 Training Week, and the captions were manually-curated by several community members.
Whenever you’re ready to get started, you can access the material by clicking on the different icons in the program!
Doing the tutorials - Technical requirements
Some of you have asked about the technical requirements. You don’t need a specific operating system or software installed, all you need is a browser and internet connection.
To run the tutorials, you will need a Galaxy account. We recommend you to:
-
Register and use European Galaxy server
You will receive an email to confirm your account before you can start using Galaxy.
-
Join the training event group, it will make your jobs go faster!
Support & Communication channels
Should you have any questions, the instructors will be available in chat. We will use the Slack space of the Galaxy Training Network. Depending on your location you might need to use a VPN, so please make sure that you can join Slack before the workshop.
Once you are in, you will see different channels (#general, #covid2021-day- for the different days) but also #random, #social. Pass by and say hi to your colleagues! Every day we will have an icebreaker question for the #social channel.
When asking a question:
- Ask in the appropriate place:
- #general for general issues
- #event-covid2021-day-1 for day 1 (Introduction to Galaxy)
- #event-covid2021-day-2 for day 2 (Data Upload & Quality Control)
- #event-covid2021-day-3 for day 3 (SARS-CoV-2 Data Analysis on Public Datasets)
- #event-covid2021-day-4 for day 4 (Visualisation & Data Export)
- Use threads.
- Say which server you’re using.
- Share all of the details (What did the tool say? What was the error? Did you see more information in the bug-report icon?)
During the week of the workshop, the instructors will be there to reply to your questions. Please be aware of the time zones, the instructors are scattered all over the world and sometimes you may have to be patient to get a reply.
Real-time Q&A sessions will be run on days 2, 3 and 4 (9 am and 4 pm CEST). Find the details to join these sessions and register by adding your name to the attendees list in the dedicated document.
Certificates
If you need a certificate, you can request it at the end of the workshop. Please make sure to keep all the work over the 4 days, stay active in the discussions and fill out the final survey.
Code of Conduct
Everyone is expected to abide by the Code of Conduct (CoC) to make this environment welcoming and friendly for everyone.
Instructors & helpers
Name | Location |
---|---|
Wolfgang Maier | Germany |
Bérénice Batut | Germany |
Beatriz Serrano-Solano | Germany |
Engy Nasr | Germany |
Simon Bray | Germany |
Florian Heyl | Germany |
Björn Grüning | Germany |
Anton Nekrutenko | USA |
Andrew Page | UK |
Carla Cummins | UK |
Peter van Heusden | South Africa |
Erik Hjerde | Norway |
Annbjørg Barbakken | Norway |
Kjell Petersen | Norway |
Steven Morgan | Australia |
Gareth Price | Australia |
Anna Syme | Australia |
Igor Makunin | Australia |
Valentine Murigneux | Australia |
Michael Thang | Australia |